Polyphosphat (PolyP) ist ein anorganisches Biopolymer, welches in allen Organismen vorkommt und daher von fundamentaler Bedeutung ist. PolyP-Granula sind komplexe Gebilde (Polyphosphatosomen), die aus einem PolyP-Kern und daran gebundenen Proteinen (z.B. PolyP-Kinasen, Phosinen etc.) bestehen. Wir untersuchen die Struktur, die Bildung und die spezifischen Funktionen von PolyP Granula mit molekularbiologischen Methoden und Life Imaging Techniken in Agrobacterium tumefaciens und Ralstonia eutropha als Modellorganismen.
Publikationen
Artikel:
Tumlirsch T, Sznajder A, Jendrossek D. 2015. Formation of polyphosphate by polyphosphate kinases and its relationship to poly(3-hydroxybutyrate) accumulation in Ralstonia eutropha H16. AEM 81:8277–93.
Tumlirsch T, Jendrossek D. 2017. Proteins with CHADs (Conserved Histidine α-Helical Domains) are attached to polyphosphate granules in vivo and constitute a novel family of polyphosphate-associated proteins (Phosins). AEM 83:e03399
Hildenbrand J C, Reinhardt S, Jendrossek D. 2019. Formation of an organic-inorganic biopolymer: polyhydroxybutyrate-polyphosphate. Biomacromolecules, DOI: 10.1021/acs.biomac.
Jüngert JR, Borisova M, Mayer C, Wolz C, Brigham CJ, Sinskey AJ, & Jendrossek D (2017). Absence of ppGpp leads to increased mobilization of intermediately accumulated poly(3-hydroxybutyrate) (PHB) in Ralstonia eutropha H16. AEM83:e00755–17
Frank C, Jendrossek D. 2020. Acidocalcisomes and polyphosphate granules are different subcellular structures in Agrobacterium tumefaciens. AEM. http://doi.org/10.1128/AEM.02759-19
Hildenbrand J C., Teleki, A., Jendrossek, D. 2020. A universal polyphosphate kinase: PPK2c of Ralstonia eutropha accepts purine and pyrimidine nucleotides including uridine diphosphate. Applied Microbiology and Biotechnology, 265(5), 11734–9.
Frank C, Teleki A, Jendrossek D. 2020. Characterization of Agrobacterium tumefaciens PPKs reveals the formation of oligophosphorylated products up to nucleosidenona-phosphates. Appl Microbiol Biotechnol (2020) 104:9683–9692; /doi.org/
Jendrossek. 2020. Polyphosphate granules and acidocalcisomes. In: Bacterial inclusions. Microbiol. Monographs. A. Seinbüchel (series ed.), D. Jendrossek (ed), 1-17; Springer, Heidelberg
Rosigkeit, H., Kneißle, L., Obruca, S., & Jendrossek, D. (2021). The Multiple Roles of Polyphosphate in Ralstonia eutropha and Other Bacteria. Microbial Physiology, 31(2), 1–15.
Frank, C., Pfeiffer, D., Aktas, M., Jendrossek, D. 2022. Migration of polyphosphate granules in Agrobacterium tumefaciens. Microbial Physiology, https://doi.org/10.1159/000521970
Hildenbrand J C, Sprenger G, Teleki A, Takors R, Jendrossek, D. 2022. Polyphosphate kinases phosphorylate thiamine phosphates, Appl Microbiol Biotechnol , DOI: 10.1159/000526662
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