Polyphosphate (PolyP) is an inorganic biopolymer that occurs in all organisms and is therefore of fundamental importance. PolyP granules are complex structures (polyphosphatosomes) consisting of a polyP core and proteins bound to it (e.g. polyP kinases, phosins, etc.). We investigate the structure, formation and specific functions of polyP granules using molecular biological methods and life-imaging techniques in Agrobacterium tumefaciens and Ralstonia eutropha as model organisms.
Publications
Articles:
Tumlirsch T, Sznajder A, Jendrossek D. 2015. Formation of polyphosphate by polyphosphate kinases and its relationship to poly(3-hydroxybutyrate) accumulation in Ralstonia eutropha H16. AEM 81:8277–93.
Tumlirsch T, Jendrossek D. 2017. Proteins with CHADs (Conserved Histidine α-Helical Domains) are attached to polyphosphate granules in vivo and constitute a novel family of polyphosphate-associated proteins (Phosins). AEM 83:e03399
Hildenbrand J C, Reinhardt S, Jendrossek D. 2019. Formation of an organic-inorganic biopolymer: polyhydroxybutyrate-polyphosphate. Biomacromolecules, DOI: 10.1021/acs.biomac.
Jüngert JR, Borisova M, Mayer C, Wolz C, Brigham CJ, Sinskey AJ, & Jendrossek D (2017). Absence of ppGpp leads to increased mobilization of intermediately accumulated poly(3-hydroxybutyrate) (PHB) in Ralstonia eutropha H16. AEM83:e00755–17
Frank C, Jendrossek D. 2020. Acidocalcisomes and polyphosphate granules are different subcellular structures in Agrobacterium tumefaciens. AEM. http://doi.org/10.1128/AEM.02759-19
Hildenbrand J C., Teleki, A., Jendrossek, D. 2020. A universal polyphosphate kinase: PPK2c of Ralstonia eutropha accepts purine and pyrimidine nucleotides including uridine diphosphate. Applied Microbiology and Biotechnology, 265(5), 11734–9.
Frank C, Teleki A, Jendrossek D. 2020. Characterization of Agrobacterium tumefaciens PPKs reveals the formation of oligophosphorylated products up to nucleosidenona-phosphates. Appl Microbiol Biotechnol (2020) 104:9683–9692; /doi.org/
Jendrossek. 2020. Polyphosphate granules and acidocalcisomes. In: Bacterial inclusions. Microbiol. Monographs. A. Seinbüchel (series ed.), D. Jendrossek (ed), 1-17; Springer, Heidelberg
Rosigkeit, H., Kneißle, L., Obruca, S., & Jendrossek, D. (2021). The Multiple Roles of Polyphosphate in Ralstonia eutropha and Other Bacteria. Microbial Physiology, 31(2), 1–15.
Frank, C., Pfeiffer, D., Aktas, M., Jendrossek, D. 2022. Migration of polyphosphate granules in Agrobacterium tumefaciens. Microbial Physiology, https://doi.org/10.1159/000521970
Hildenbrand J C, Sprenger G, Teleki A, Takors R, Jendrossek, D. 2022. Polyphosphate kinases phosphorylate thiamine phosphates, Appl Microbiol Biotechnol , DOI: 10.1159/000526662
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